The objective of this study was to examine the effects of antioxidant vitamins on the cellular oxidant damage by observing the mitogenicity in the mouse spleen and the strand breaks of DNA in mouse blood induced by AFB1.
Intraperitoneal(i.p.)
injections of vitamin C(VC) of 10 ㎎/㎏ and vitamin E(VE) of 63.8 ㎎/㎏ were repeatedly administered to male ICR mice of 6 weeks old at intervals of 4 times every 2 days. After one hour vitamin treatments, AFB1 of 0.4 ㎎/㎏ was
injected
into
the AFB1 plus vitamin treated groups in the same way. On the other hands, into the AFB1 only trated group, only AFB1 was injected without vitamins in the same method as above. The results of the experiment are as
follows
; as regard to comet assay, DNA strand breaks were clearly present and they formatted a typical comet tail in the mice blood of the AFB1 only treated goups. However, comet tails apparently disappeared in AFB1 plus
antioxidant
vitamins treated groups since oxidant damage was controlled in an almost similar level to the control group. Mitogenicity of the spleen also showed a similar tendency as before, and these differences were more remarkably observed in the reaction
against
Con-A, which is a T-cell mitogen. In these data, the statistical significance was p<0.01. The LDL and VLDL levels were 408.72, 504.47 ㎎/㎗ respectively in the AFB1 only treated groups. Compared with the AFB1 only treated
groups,
those of AFB1 plus antioxidant vitamin treated groups decreased to 272.06(VC), 305.28 ㎎/㎗(VE), respectively. On the other hand, HDL levels were diminished to 32.60, 29.60 ㎎/㎗ in AFB1 only treated groups, compared to
42.23,
41.14 ㎎/㎗ in the AFB1 plus antioxidant vitamins treated groups. But, blood glucose levels were not statistically significant.
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